Title:Efficient Bulked Approaches to Map Trait-associated Genomic Variation
Reporter:Liu Sanzhen (Kansas State University)
Organizer:State Key Laboratory of Tree Genetics and Breeding
Time:8:50 a.m., June 5, 2017 (Monday)
Venue:Room 104, Main Building
Participants:all teachers and students
About the Report
The development of next-generation sequencing technology provides a powerful research tool for many biology research areas. Applications for numerous branches have been rapidly developed to help solve the related biological problems, based on the next-generation sequencing platform. This report will introduce several research examples to explore how to effectively use next-generation sequencing techniques, including how to quickly map plant trait genes using sequencing, and how to assemble the complete genome using long sequence sequencing. First, gene cloning of corn glossy will be used as an example to introduce the rapid and effective RNA sequencing to map single gene-controlled trait gene (BSR-Seq). Second, corn bacterial disease (Goss’s wilt) will be taken as an example to analyze complex genetic traits by using the XP-GWAS and the XP-CNV. In addition, genomic sequencing and assembly of pathogens such as Goss's wilt will be briefly discussed.
About the Reporter
Liu Sanzhen is the professor at Kansas State University at present. After obtaining the bachelor and master degree at Xiamen University in 1997 and 2000 respectively, Dr. Liu obtained his Ph.D. degree in genetics from Iowa University in the United States in 2010.After serving as a post-doctoral research associate atIowa Universityfrom 2010-2013, he served as a professor in Department of Plant Pathology, Iowa University in 2013.
Representative Papers
Professor Liu has published more than 30 SCI journal papers in PloS Genetics, Plant Journal, Science, Nature Communication, etc. His research interests include: (1) corn and wheat resistance to various pathogens; (2) the phenotypic consequence of genomic structural variation; (3) mRNA variable splicing; (4) adaption of cutting-edge genomic technologies to facilitate plant genetic and genomic studies.
